Composite

Part:BBa_K5117020

Designed by: Jenny Sauermann   Group: iGEM24_TU-Dresden   (2024-08-31)


BsRBS-BhrPET

This part serves as translational unit composed of the ribosome binding site of Bacillus subtilis (BBa_K5117000) and the gene of the uncultured bacterium HR29 (BBa_K5117010) encoding a polyethylene terephthalate hydrolase (PETase, EC 3.1.1.101), codon optimized for Bacillus subtilis (Xi et al. 2021) and including a secretory signal peptide SPaprE.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 594
    Illegal AgeI site found at 639
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Xi X., Ni K., Hao H., Shang Y., Zhao B., Qian Z. (2021): Secretory expression in Bacillus subtilis and biochemical characterization of a highly thermostable polyethylene terephthalate hydrolase from bacterium HR29. Enzyme and microbial technology 143, 109715.


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