Composite
Part:BBa_K5117020
Designed by: Jenny Sauermann Group: iGEM24_TU-Dresden (2024-08-31)
BsRBS-BhrPET
This part serves as translational unit composed of the ribosome binding site of Bacillus subtilis (BBa_K5117000) and the gene of the uncultured bacterium HR29 (BBa_K5117010) encoding a polyethylene terephthalate hydrolase (PETase, EC 3.1.1.101), codon optimized for Bacillus subtilis (Xi et al. 2021) and including a secretory signal peptide SPaprE.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 594
Illegal AgeI site found at 639 - 1000COMPATIBLE WITH RFC[1000]
References
Xi X., Ni K., Hao H., Shang Y., Zhao B., Qian Z. (2021): Secretory expression in Bacillus subtilis and biochemical characterization of a highly thermostable polyethylene terephthalate hydrolase from bacterium HR29. Enzyme and microbial technology 143, 109715.
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Categories
Parameters
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